Siri Eldevik Håberg MD PhD I4C, Lyon, November 12, 2012.

Presentasjon om: "Siri Eldevik Håberg MD PhD I4C, Lyon, November 12, 2012."— Utskrift av presentasjonen:

1 Siri Eldevik Håberg MD PhD I4C, Lyon, November 12, 2012

2 The Norwegian Mother & Child Cohort Study Recruitment Hospitals with < 1000 births/year Hospitals with >1000 births/year Norway  60,000 births/year Inclusion period 1999 - 2009

3 The cohort Number Women91 000 Men72 000 Children109 000 Pregnancies107 000 Twin-pairs1 870 Triplet trios 21 Sibships~ 15 000

4 Data collection Mother Father Child Cord blood Ultrasound Week 18Week 30Birth6 months18 months 3, 5, 7,8 years (12/13) Blood Teeth

5 Inclusion 1999 – 2009 (last birth in 2010)

6 Biological samples MoBa: Top modern biobank – automated/manual •EDTA fullblood and plasma (frozen -86 o C) •DNA extracted from fullblood (frozen -20 o C) •Urine(frozen -20 o C ) •RNA: from cord blood (Tempus-tubes) •Milk teeth

7 MoBa I4C Cancer •National registries linked to MoBa –Birth registry –Cancer registry (I4C) –Prescription registry –Patient registry (hospitals) –Vaccination registry –… –Specified variables (can be extended)

8 Epigenetic substudy •An asthma project within MoBa –Collaboration NIEHS (USA) /NIPH(Norway) •4000 maternal samples –B vitamin metabolites (around 20 - folate etc) –SNPs in B-vitamin metabolism (around 20) –2000 have A,D,E vitamin measured •1050 (+750) children of these mothers have Illumina 450 K methylation data –(cord blood DNA)

9 Epigenetic findings/plans •Identified 10 genes with methylation changes present at birth in children whose mothers smoked during pregnancy –(AHRR, CYP1A1, GFI1…..) •Studies in progress: –Birth weight, asthma,… –Leukemia in children (Danish/Norwegian pilot) –other exposures/outcomes (vitamins, diet, asthma..)

10 Thank you

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12 Kvalitetsoppgaver i vanlig drift Prosedyre for DNA: DNA ekstraksjon•DNA isoleres fra blod. Fordeling•Fortynnes til 100 ng/µl. •Fordeles til fem 96 brønners brett og lagres ved -20 ºC.

13 DNA ekstraksjon Etter DNA ekstraksjon: •Brune pelleter tas ut og ekstraheres på nytt •Eventuelle avvik kommenteres i MoBaStudy

14 DNA fordeling OD måling: konsentrasjon og 260/280 ratio •Konsentrasjon under 100 ng/µl bemerkes i MoBaStudy. •Konsentrasjon over 145 ng/µl fortynnes og OD måling gjentas. •Alle spesialtilfeller kommenteres i MoBaStudy. •Prøver med en 260/280 ratio utenfor 1,6-2,0. kommenteres i programmet, og isoleres på nytt. •Hver 12. prøve inkluderes i kvalitetskontrollprogrammet.